Laboratory Report 151073-407

Evaluation of One Formulation When Applied to a Wounded Full-Thickness Tissue Model in Two Different Methods of Application to Evaluate Antimicrobial Activity

SPONSOR
Human Health Foundation, LLC, 742 Goddard Avenue, Chesterfield, Missouri 63005

TESTING FACILITY
BioScience Laboratories, Inc.,  1755 South 19th Avenue,  Bozeman, Montana 59718

STUDY INITIATION DATE: 01/15/16
STUDY COMPLETION DATE: 04/21/16

An evaluation of the antimicrobial properties of two applications of one test material, Elastic Skin Liquid Bandage (Lot #Not Provided), was performed versus Acinetobacter baumannii (ATCC #19606), methicillin resistant Staphylococcus aureus (MRSA, ATCC #43300), and Streptococcus pyogenes (ATCC #19615) using a wounded full thickness epidermal tissue model, EpiDerm FT™. Twenty-five wounded tissues were contaminated with their designated challenge strain, incubated for one hour to allow for bacterial attachment, and rinsed with PBS (Phosphate Buffered Saline) to remove unattached bacteria. Five tissues were used as a negative (sterility) control for each test day. Test formulation applications were performed as follows: 1) Application A: Each of five wounded tissues was inoculated with 0.1 mL of the test formulation and exposed to either 5, 15, or 30 minutes; 2) Application B: Each of five wounded tissues were irrigated with l .OmL of the test formulation and aspirated immediately. Five wounded tissues remained untreated and served as positive (inoculum) controls. The percent and Log10 reductions in the challenge strain population from each of the test tissues was compared to the average population recovered from the untreated inoculum control tissues. Tables 1-3 summarize the results for each challenge strain versus the two product applications of the test material.

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Lab Study 151073-407 Table 1
Table 1
Lab Study 151073-407 Table 2
Table 2
Lab Study 151073-407 Table 1
Table 3
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